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A structure-based assignment of the glutathione activator site without transition-state analogues is much more difficult because of labile GSH interactions 2 that likely depend not only on the presence of the first glutathione moiety but also on conformational changes of the glutathionylated enzyme 3,12,37,44

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Key points This is a protocol for the preparation and characterization of the activatable BTPE-NO 2 @F127 nanoprobe and its applications in multispectral optoacoustic and near-infrared region II fluorescence dual-mode imaging of liver injury models in mice

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