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Similarly, no changes in the folate content were observed in eggs stored at refrigerator temperature (47 C) or room temperature (1820 C) for 27 days (i.e., from the date of laying to the best before date)

PVDF membranes after blocking were incubated with primary 6His tag monoclonal antibody (1:1,000 dilution) and GAPDH loading control monoclonal antibody (1:2,500 dilution) at 4 C overnight

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