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The reaction was initiated by the addition of 190 l reaction buffer, adjusted to pH 7.4 by titration with K 2 HPO 4 (40 mM) and KH 2 PO 4 (10 mM) stock solutions, supplemented with NADH (100 M) and sodium pyruvate (600 M)

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The JAK/STAT signaling pathway, as the main target for inhibiting the action of a variety of cytokines (221), is involved in the signal transduction of many key cytokines for the pathogenesis of RA and has been considered as a potential target for the treatment of RA (222)