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Continuous, daily usage without breaks is generally not recommended due to the lack of extensive human data and potential risks 1 2

The effects of D2R-DAT disruption in DA-depleted animals We next examined whether dopamine depletion can affect the ability of TAT-DAT NT to promote locomotor behavior

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Lack of sleep also makes you hungrier, causes you to store more fat, and makes your body less sensitive to the hormone insulin, sabotaging attempts to lose weight and increasing your risk of developing type 2 diabetes or cardiovascular disease