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2 Materials and methods A comprehensive search was conducted on the medical databases, including Web of Science, PubMed, China National Knowledge Infrastructure (CNKI), Wanfang Data, and VIP Database, using terms such as Bovis Calculus natural Bovis Calculus Bovis Calculus artifactus Bovis Calculus sativus cerebral ischemia ischemic stroke traditional applications chemical components clinical applications clinical safety pharmacokinetic characteristics safety evaluation

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Kansanen E, Bonacci G, Schopfer FJ, Kuosmanen SM, Tong KI, Leinonen H, et al

The pQE-30 bacterial expression plasmid of the U46C mutant of human c-GPX4 was mutated to obtain the D23A mutant

Andreeff M , Young C, Clarkson B, Fetten J, Rifkind R, Marks P