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At the tissue level, the exposure led to pronounced metabolic imbalance, with increased oxygen consumption and ammonia excretion, changes in the activity of liver enzymes, oxidative stress, structural damage to the liver, gills, intestines, skin and muscles, suppression of acetylcholinesterase activity in the brain, and altered expression of genes of the reproductive axis (Yong, Valiyaveetill & Tang, 2020)