Use sterile technique, wipe the stopper before each access, avoid touching sterile surfaces, and keep the vial refrigerated when not in use
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China Patent CN102492591 (B) Xiong Z, Guo M, Guo Y, Chu J, Zhuang Y, Zhang S (2008) Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium