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Moreover, rapamycin upregulated Wnt/-catenin signaling, including statistically significant upregulation of the T cell memory genes TCF7, LEF1, FOXO1 in rapamycin-treated products (Supplementary Data 5b)

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The continuous use approach represents traditional treatment strategy where daily GHK-Cu application continues indefinitely or until goals achieved, then potentially reduces to maintenance frequency (every other day or few times weekly) rather than complete discontinuation