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NAC induced only slight morphological alterations, specifically an increase in granularity at the highest concentration tested (Additional file 1: Figure S3)

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2 Introduction Blood is a multifaceted body fluid and the medium through which essential nutrients are delivered to tissues throughout the body

Followed by SDS-PAGE electrophoresis, the transfer of proteins was conducted onto PVDF (Millipore) membranes (Solebio, Beijing, China) and immunocontainment experiments were performed after the membrane transfer was completed, the primary antibody was added and incubated at 4 C overnight with slow shaking

The availability of this control probe is a key advantage of our sensor over existing probes, providing an excellent way to evaluate changes in redox homeostasis in live cells